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. 1982 Jul 1;156(1):312-7.
doi: 10.1084/jem.156.1.312.

Restricted nucleocytoplasmic relationship in activation of T and B lymphocytes

T Watanabe, Y Eda, J Ohara

Restricted nucleocytoplasmic relationship in activation of T and B lymphocytes

T Watanabe et al. J Exp Med. .

Abstract

Nuclei of murine T lymphocytes or B lymphocytes were purified and transferred into lethally irradiated whole spleen cells or B or T lymphocytes by means of polyethyleneglycol-mediated cell fusion. Transfer of lymphocyte nuclei could save the irradiated cells from cell death, and such reconstituted cells could respond to mitogens. The present study showed that nuclei of T cells could be activated in the concanavalin A-stimulated T cell cytoplasms but not in the lipopolysaccharide-stimulated B cell cytoplasms. On the other hand, nuclei of B cells were activated in the lipopolysaccharide-stimulated B cells but not in the concanavalin A-stimulated T cell cytoplasms. These data suggested that a specific interaction between cytoplasm and nucleus might exist in the activation of nuclei of each lymphocyte subset.

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