Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Aug 1;205(2):413-7.
doi: 10.1042/bj2050413.

The interaction of anthraquinone dyes with the plasmid-mediated OXA-2 beta-lactamase

C Monaghan, S Holland, J W Dale

The interaction of anthraquinone dyes with the plasmid-mediated OXA-2 beta-lactamase

C Monaghan et al. Biochem J. .

Abstract

Although beta-lactamases do not require any nucleotide co-substrates, the OXA-2 type is inhibited competitively by Cibacron Blue 3GA, and by other anthraquinone dyes, including some simpler compounds with no side chain. The enzyme causes a red shift in the spectrum of Cibacron Blue. The beta-lactamase can be adsorbed in Blue Sepharose and specifically eluted by benzylpenicillin. These results indicate that the binding of anthraquinone dyes is a specific effect similar to that seen with many nucleotide-binding enzymes.

PubMed Disclaimer

References

    1. Biochem J. 1971 Jul;123(4):493-500 - PubMed
    1. Biochem J. 1971 Jul;123(4):501-5 - PubMed
    1. J Bacteriol. 1974 Aug;119(2):351-6 - PubMed
    1. Anal Biochem. 1975 Jan;63(1):17-26 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Feb;72(2):669-72 - PubMed

Publication types