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. 1982 Nov;79(21):6617-21.
doi: 10.1073/pnas.79.21.6617.

T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood

R J Albertini, K L Castle, W R Borcherding

T-cell cloning to detect the mutant 6-thioguanine-resistant lymphocytes present in human peripheral blood

R J Albertini et al. Proc Natl Acad Sci U S A. 1982 Nov.

Abstract

Rare thioguanine-resistant T lymphocytes, present in vivo in human peripheral blood, were isolated and grown in vitro as thioguanine-resistant cultured T cells. The conditions for their selection in vitro were such that thioguanine resistance had to have arisen in vivo. The mutant cells bore T-cell surface markers, maintained their thioguanine resistance in vitro in the presence or absence of selection, and were deficient in hypoxanthine-guanine phosphoribosyltransferase activity.

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