[Determination of T- and B-lymphocyte counts in guinea pigs]

Abstract

T lymphocytes of guinea-pigs were found to form spontaneous E-rosettes with rabbit red blood cells. The lymphocyte count constituted 32% of blood lymphocytes, 41% of lymph node cells. Meanwhile no T lymphocytes were detected in the bone marrow. The T lymphocyte subpopulation capable of forming "early" rosettes was demonstrated. B lymphocytes formed EAC-rosettes with sheep red blood cells. The B lymphocyte count in the blood, lymph nodes and bone marrow amounted to 10, 18 and 5%, respectively. The effects of incubation conditions, centrifugation, antibody types, and complement concentration, etc. on E- and EAC-rosette formation were defined.