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. 1982 Dec;363(12):1493-500.
doi: 10.1515/bchm2.1982.363.2.1493.

Isolation of a high molecular mass granulocyte colony-stimulating factor from bovine lung conditioned medium

R Neumeier, H R Maurer

Isolation of a high molecular mass granulocyte colony-stimulating factor from bovine lung conditioned medium

R Neumeier et al. Hoppe Seylers Z Physiol Chem. 1982 Dec.

Abstract

A high molecular mass type of granulocyte colony-stimulating factor (CSF-F) from bovine lung tissue produced by fibroblasts was isolated and partially purified by a four-step procedure. Following ammonium sulfate fractionation, hydrophobic interaction, ion-exchange and affinity chromatography, CSF-F was more than 200-fold enriched. CSF-F was found to be a glycoprotein of approximately 68 kDa with an isoelectric point of 4.0-5.2. It did not lose its biological activity after neuraminidase treatment. Gel-filtration behavior of desialo-CSF-F was not changed.

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