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. 1980 Jan;141(1):41-51.
doi: 10.1128/jb.141.1.41-51.1980.

Polarity of amber mutations in ribosomal protein genes of Escherichia coli

T Cabezón, G Delcuve, M Faelen, L Desmet, A Bollen

Polarity of amber mutations in ribosomal protein genes of Escherichia coli

T Cabezón et al. J Bacteriol. 1980 Jan.

Abstract

Two amber mutations have been mapped inside the spcA-strA region (now called rpsE-rpsL) on the bacterial genome. Derivatives of the transducing phage lambda fus3 carrying each mutation were constructed and assayed in ultraviolet-irradiated bacteria to identify the mutated genes and measure the polarity of the mutations. The data indicated that both mutations, 3162(Am) and 3161(Am), affect genes coding for ribosomal proteins: rplC (L3) and rpsN (S14), respectively. It was shown also that each mutation exerts, inside of its respective operon (S10 and spc units), a relatively strong polar effect on genes distal to the mutated locus.

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