Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Mar;28(3):276-8.
doi: 10.1177/28.3.6986437.

Back to back autoradiography: a method for ultrastructural analysis of light microscopic autoradiographs

N B Kaplan, L J Smith, J S Brody

Back to back autoradiography: a method for ultrastructural analysis of light microscopic autoradiographs

N B Kaplan et al. J Histochem Cytochem. 1980 Mar.

Abstract

A method for studying radiolabeled cells is described that combines the simplicity of light microscopic autoradiography with the high resolution of electron microscopy. Serial thin (600 A) and thick (1 micron) sections are placed on Formvar-coated slot grids and glass slides, respectively. Labeled cells are visualized on the thick section by autoradiography and may then be studied in the electron microscope by locating the corresponding fields on the grid. This technique permits accurate ultrastructural identification and analysis of radiolabeled cells, yet avoids the need for electron microscopic autoradiography.

PubMed Disclaimer

Publication types

LinkOut - more resources