doi: 10.1093/nar/8.1.143.
Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methylgroups on ribosomal subunit interaction
- PMID: 6986611
- PMCID: PMC327248
- DOI: 10.1093/nar/8.1.143
Item in Clipboard
Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16S ribosomal RNA of Escherichia coli. IV. The effect of the methylgroups on ribosomal subunit interaction
Nucleic Acids Res.
.
Abstract
The effect of the presence or absence of the methylgroups of the m2(6)Am2(6)A sequence near the 3' end of 16S rRNA of Escherichia coli on the interaction of the ribosomal subunits has been studied, using wild-type (methylated) and mutant (unmethylated) ribosomes. Subunit exchange experiments and competitive association experiments show a strong preference of the 50S subunit for association with methylated 30S subunits. The results indicate that the equilibrium constant of the reaction 70S in equilibrium with 30S + 50S is dependent on the methylgroups; mutant 30S.50S couples are less stable than wild-type 30S.50S couples. It is postulated that the methylgroups also stimulate the interaction between 30S subunits and initiation factor IF-3.
Similar articles
-
Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. II. The effect of the absence of the methyl groups on initiation of protein biosynthesis.J Biol Chem. 1979 Sep 25;254(18):9090-3. J Biol Chem. 1979. PMID: 383711
-
Magnesium-dependent interaction of 30S ribosomal subunits with antibodies to N6, N6-dimethyladenosine.Biochemistry. 1980 Aug 5;19(16):3786-91. doi: 10.1021/bi00557a021. Biochemistry. 1980. PMID: 6996711
-
Binding of S21 to the 50S subunit and the effect of the 50S subunit on nonradiative energy transfer between the 3' end of 16S RNA and S21.Biochemistry. 1984 Oct 9;23(21):5069-76. doi: 10.1021/bi00316a037. Biochemistry. 1984. PMID: 6388639
-
In vitro methylation of Escherichia coli 16S ribosomal RNA and 30S ribosomes.Proc Natl Acad Sci U S A. 1989 Jul;86(13):4902-6. doi: 10.1073/pnas.86.13.4902. Proc Natl Acad Sci U S A. 1989. PMID: 2662188 Free PMC article.
-
Intermolecular base-paired interaction between complementary sequences present near the 3' ends of 5S rRNA and 18S (16S) rRNA might be involved in the reversible association of ribosomal subunits.Nucleic Acids Res. 1979 Dec 11;7(7):1913-29. doi: 10.1093/nar/7.7.1913. Nucleic Acids Res. 1979. PMID: 94160 Free PMC article. Review.
Cited by
-
Structural basis for binding of RNA and cofactor by a KsgA methyltransferase.Structure. 2009 Mar 11;17(3):374-85. doi: 10.1016/j.str.2009.01.010. Structure. 2009. PMID: 19278652 Free PMC article.
-
The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3' end of 16S rRNA.Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10156-61. doi: 10.1073/pnas.1017679108. Epub 2011 Jun 6. Proc Natl Acad Sci U S A. 2011. PMID: 21646538 Free PMC article.
-
Relative abundance of the human mitochondrial transcription system and distinct roles for h-mtTFB1 and h-mtTFB2 in mitochondrial biogenesis and gene expression.Nucleic Acids Res. 2007;35(12):4042-54. doi: 10.1093/nar/gkm424. Epub 2007 Jun 8. Nucleic Acids Res. 2007. PMID: 17557812 Free PMC article.
-
A single base change in the Shine-Dalgarno region of 16S rRNA of Escherichia coli affects translation of many proteins.Proc Natl Acad Sci U S A. 1987 Jul;84(14):4757-61. doi: 10.1073/pnas.84.14.4757. Proc Natl Acad Sci U S A. 1987. PMID: 2440027 Free PMC article.
-
Structural and catalytic roles of the human 18S rRNA methyltransferases DIMT1 in ribosome assembly and translation.J Biol Chem. 2020 Aug 21;295(34):12058-12070. doi: 10.1074/jbc.RA120.014236. Epub 2020 Jul 2. J Biol Chem. 2020. PMID: 32616653 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
