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. 1980 Feb;65(2):143-8.
doi: 10.1007/BF00493162.

Immunohistochemical localization of alkaline phosphatase in the chicken epiphyseal growth cartilage

Immunohistochemical localization of alkaline phosphatase in the chicken epiphyseal growth cartilage

H K Väänänen. Histochemistry. 1980 Feb.

Abstract

Alkaline phosphatase of chicken epiphyseal cartilage has been localized by two immunohistochemical methods. Double layer immunofluorescence and peroxidase anti-peroxidase (PAP) methods gave similar results. Alkaline phosphatase in epiphyseal cartilage is extracellular as well as intracellular in the localization. Extracellular reaction was strongest in the lower layers of growth plate and the most intense reaction was noted in the pericellular lacunae of hypertrophic chondrocytes. Also intracellular immunoreaction was noticed through the whole growth plate.

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