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. 1980 Feb 28;283(5750):835-40.
doi: 10.1038/283835a0.

Abnormal expression of chromosomal rabbit beta-globin gene in Saccharomyces cerevisiae

J D Beggs, J van den Berg, A van Ooyen, C Weissmann

Abnormal expression of chromosomal rabbit beta-globin gene in Saccharomyces cerevisiae

J D Beggs et al. Nature. .

Abstract

A 5.1 kilobase-pair segment of rabbit chromosomal beta-globin DNA was joined to pJDB219, a plasmid consisting of pMB9, the 2-mu yeast plasmid and the yeast leu-2 gene. Saccharomyces cerevisiae transformed with the globin DNA-containing hybrid produced beta-globin-specific RNA. As compared to mature beta-globin mRNA, these transcripts lacked 20-40 nucleotides from the 5' end, contained all of the small intron and extended to about the middle of the large intron. Thus, no splicing of the primary beta-globin transcript could be detected in the yeast cells.

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