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. 1980 Mar 27;284(5754):316-20.
doi: 10.1038/284316a0.

Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

S Nagata, H Taira, A Hall, L Johnsrud, M Streuli, J Ecsödi, W Boll, K Cantell, C Weissmann

Synthesis in E. coli of a polypeptide with human leukocyte interferon activity

S Nagata et al. Nature. .

Abstract

Double-stranded cDNA prepared from the 12S fraction of poly(A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clones had a 910-base pair insert which could hybridise to IF mRNA and was responsible for the production of a polypeptide with biological IF activity. Up to 10,000 units IF activity per g of cells was obtained from some clones.

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