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. 1980 Jan;177(2):193-7.
doi: 10.1007/BF00267429.

Synthesis of P1 ban protein in minicells infected by P1 mutants

J N Reeve, E Lanka, H Schuster

Synthesis of P1 ban protein in minicells infected by P1 mutants

J N Reeve et al. Mol Gen Genet. 1980 Jan.

Abstract

Phage P1 encodes a dnaB analog (ban) protein. Synthesis of ban protein has been studied in minicells infected by P1 mutants and has been identified as a polypeptide of 56,000 molecular weight by immunoprecipitation using antibody directed against E. coli dnaB protein. The amount of ban protein synthesized by P1 mutants increases in the order: P1 wild type, P1bac, P1crr, and P1bac crr. The relative amount of ban protein identified in P1bac- and P1bac crr-infected minicells is approximately the same as that previously found in dnaB.ban heteromultimers isolated from the corresponding P1 lysogens.

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