A simplified method of DNA distribution analysis

Abstract

Current methods of analysing DNA distributions utilize complex mathematical expressions that require the use of large non-linear curve fitting methods and, consequently, large computers. This paper presents a new method of analysing DNA distributions of asynchronously growing or mildly perturbed cells. The S phase fraction is obtained by fitting a second degree polynomial to that part of the distribution, mid S phase, which is not influenced by either the G1 or the G2 + M peaks. The method is simple and fast, it exceeds the accuracy of other methods and it can be used on a large desk calculator or mini-computer.