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. 1980 Apr;142(1):120-30.
doi: 10.1128/jb.142.1.120-130.1980.

Genetic locus, distant from ptsM, affecting enzyme IIA/IIB function in Escherichia coli K-12

R A Roehl, R T Vinopal

Genetic locus, distant from ptsM, affecting enzyme IIA/IIB function in Escherichia coli K-12

R A Roehl et al. J Bacteriol. 1980 Apr.

Abstract

Most strains of Escherichia coli K-12 are unable to use the enzyme IIA/IIB (enzyme IIMan) complex of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in anaerobic growth and therefore cannot utilize glucosamine anaerobically. Introduction into these strains of a ptsG mutation, which eliminates activity of the enzyme IIIGlc/IIB' complex of the PTS, resulted in inability to grow anaerobically on glucose and mannose. Derivative strains able to grow anaerobically on glucosamine had mutations at a locus close to man, the gene coding for phosphomannose isomerase, and had higher enzyme IIA/IIB activities during anaerobic growth than did the parental strain. These results establish a locus affecting function of enzyme IIA/IIB that maps distant from ptsM, the probable structural gene for enzyme IIB.

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