[Immobilization of acid proteinase from Aspergillus awamori on inorganic carriers with carboxylic groups]

Abstract

To achieve acid proteinase immobilization on inorganic carriers with carboxylic groups, water soluble carbodiimides and N-carbethoxy-2-ethoxy-1,2-dihydrochinoline were used. The influence of different concentrations of the native enzyme and carbodiimides on the immobilization process was studied. The preparations of immobilized acid proteinase were obtained. Its specific activity was 73% in relation to initial activity of enzyme. Amount of binding protein was 31% and amount of binding activity was 23%. Temperature optimum of immobilized proteinase was 40-45 degrees, termostability was than immobilized enzyme.