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. 1978 Oct;24(10):1674-9.

Automated metabolic profiling of organic acids in human urine. I. Description of methods

S C Gates, N Dendramis, C C Sweeley
  • PMID: 699272

Automated metabolic profiling of organic acids in human urine. I. Description of methods

S C Gates et al. Clin Chem. 1978 Oct.

Abstract

We describe a complete procedure for separation and mechanized analysis of organic acids in human urine. The acid fraction of urine is separated by anion-exchange chromatography on diethylaminoethyl-Sephadex. Individual acids are identified and measured by use of a gas-chromatography/mass spectrometer/computer system that can clearly distinguish contributions from at least 150 substances in a single sample. We discuss analytical recoveries, contributions from the sample separation process, stability of stored samples before and after processing, and reproducibility of the extraction procedure.

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