Antibodies against Aspergillus fumigatus II. Identification and quantification by means of crossed immunoelectrophoresis

Abstract

Individual antigenic components of Aspergillus fumigatus were identified by crossed immunoelectrophoresis (CIE) using pooled rabbit hyperimmune antiserum. Patients' sera with enhanced precipitins against A. fumigatus showed antibodies against identical antigenic components in CIE. Identification of the antigenic components by the intermediate gel technique showed high concentrations of antibodies against a small group of antigenic components. No differences were found in the individual antigenic components involved in the precipitation reactions between groups of sera with enhanced and normal IgE levels against A. fumigatus. Crossed immunoelectrofocusing was used to determine the isoelectric point of particular components that were identified by CIE.