doi: 10.1073/pnas.77.5.2434.
Structural studies of methyl-accepting chemotaxis proteins of Escherichia coli: evidence for multiple methylation sites
- PMID: 6994098
- PMCID: PMC349413
- DOI: 10.1073/pnas.77.5.2434
Item in Clipboard
Structural studies of methyl-accepting chemotaxis proteins of Escherichia coli: evidence for multiple methylation sites
Proc Natl Acad Sci U S A.
1980 May.
Abstract
Two-dimensional analysis of tryptic peptides from [35S]methionine-labeled methyl-accepting chemotaxis proteins, MCP I and MCP II, demonstrates a high degree of homology between the two proteins. After the methylation sites were labeled with S-adenosyl-L-methyl-3H]methionine, peptides of three distinct migrations in each protein were found to carry a methyl group. These multiple methylations appear to be responsible in part for the observed multiple banding patterns on sodium dodecyl sulfate/polyacrylamide slab gels.
Similar articles
-
Methyl-accepting chemotaxis proteins of Escherichia coli: methylated at three sites in a single tryptic fragment.Biochemistry. 1981 Feb 17;20(4):977-82. doi: 10.1021/bi00507a051. Biochemistry. 1981. PMID: 7011380
-
The methyl-accepting chemotaxis proteins of Escherichia coli. Identification of the multiple methylation sites on methyl-accepting chemotaxis protein I.J Biol Chem. 1982 Sep 10;257(17):10378-86. J Biol Chem. 1982. PMID: 6213619
-
Multiple methylation of methyl-accepting chemotaxis proteins during adaptation of E. coli to chemical stimuli.Cell. 1980 May;20(1):165-71. doi: 10.1016/0092-8674(80)90244-5. Cell. 1980. PMID: 6993007
-
Integral membrane proteins required for bacterial motility and chemotaxis.Symp Soc Exp Biol. 1982;35:123-37. Symp Soc Exp Biol. 1982. PMID: 6764039 Review.
-
Bacterial chemotaxis and the molecular logic of intracellular signal transduction networks.Annu Rev Biophys Biophys Chem. 1991;20:109-36. doi: 10.1146/annurev.bb.20.060191.000545. Annu Rev Biophys Biophys Chem. 1991. PMID: 1867712 Review. No abstract available.
Cited by
-
Cloning of trg, a gene for a sensory transducer in Escherichia coli.J Bacteriol. 1982 Oct;152(1):372-83. doi: 10.1128/jb.152.1.372-383.1982. J Bacteriol. 1982. PMID: 6749811 Free PMC article.
-
Genetic and biochemical properties of Escherichia coli mutants with defects in serine chemotaxis.J Bacteriol. 1980 Dec;144(3):1048-60. doi: 10.1128/jb.144.3.1048-1060.1980. J Bacteriol. 1980. PMID: 6777365 Free PMC article.
-
Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: organization of the tar region.J Bacteriol. 1983 Aug;155(2):565-77. doi: 10.1128/jb.155.2.565-577.1983. J Bacteriol. 1983. PMID: 6307970 Free PMC article.
-
Evidence for methyl group transfer between the methyl-accepting chemotaxis proteins in Bacillus subtilis.J Bacteriol. 1988 Jan;170(1):223-7. doi: 10.1128/jb.170.1.223-227.1988. J Bacteriol. 1988. PMID: 3121584 Free PMC article.
-
Multiple electrophoretic forms of methyl-accepting chemotaxis proteins generated by stimulus-elicited methylation in Escherichia coli.J Bacteriol. 1980 Aug;143(2):809-15. doi: 10.1128/jb.143.2.809-815.1980. J Bacteriol. 1980. PMID: 6782079 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous
