The bovine lymphoid system. III. A monoclonal antibody specific for bovine cell surface and serum IgM
- PMID: 7000680
- PMCID: PMC1458052
Item in Clipboard
The bovine lymphoid system. III. A monoclonal antibody specific for bovine cell surface and serum IgM
Immunology.
1980 Jul.
Abstract
Mouse spleen cells from animals immunized with bovine peripheral blood lymphocytes were fused to X63 . Ag8 myeloma cells and the activity of one of the resulting myeloma hybrids was characterized. The product of this clone (B5/4.1.4) binds to pentameric bovine IgM isolated from serum but not to serum IgG1 or IgG2. This reagent also binds to cell surface (monomeric) IgM and can be used in immunofluorescence assays to enumerate IgM-bearing cells in lymphoid cell suspensions and to examine B lymphocytes or B lymphocyte derived cells in tissue sections.
Similar articles
-
Use of a monoclonal antibody specifically non-reactive with T cells to delineate lymphocyte subpopulations.Immunology. 1981 Mar;42(3):371-8. Immunology. 1981. PMID: 7009413 Free PMC article.
-
Expression of complement receptor 2 (CD21), membrane IgM and the inhibitory receptor CD32 (FcgammaRIIb) in the lymphoid tissues of neonatal calves.Vet Immunol Immunopathol. 2010 Sep 15;137(1-2):99-108. doi: 10.1016/j.vetimm.2010.04.016. Epub 2010 May 21. Vet Immunol Immunopathol. 2010. PMID: 20488561
-
Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. I. A hybridoma secreting antibody against a marker specific for human B lymphocytes.Clin Exp Immunol. 1980 Feb;39(2):477-85. Clin Exp Immunol. 1980. PMID: 6966995 Free PMC article.
-
Whither monoclonal antibodies?Immunology. 1980 Jul;40(3):287-93. Immunology. 1980. PMID: 6253387 Free PMC article. Review. No abstract available.
-
Immunoglobulin as a differentiation and clonal marker.J Immunol Methods. 1983 Dec 16;65(1-2):1-25. doi: 10.1016/0022-1759(83)90300-9. J Immunol Methods. 1983. PMID: 6361138 Review. No abstract available.
Cited by
-
Proliferation and lymphocyte stimulatory capacity of Theileria-infected lymphoblastoid cells before and after the elimination of intracellular parasites.Immunology. 1981 Sep;44(1):51-60. Immunology. 1981. PMID: 6792058 Free PMC article.
-
Identification of isotypes and allotypes of bovine immunoglobulin M with monoclonal antibodies.Immunology. 1988 Apr;63(4):569-74. Immunology. 1988. PMID: 3259201 Free PMC article.
-
Monoclonal antibodies which react with bovine T-lymphocyte antigens and induce blastogenesis: tissue distribution and functional characteristics of the target antigens.Immunology. 1988 Mar;63(3):439-46. Immunology. 1988. PMID: 2965099 Free PMC article.
-
The properties and functional activity of non-lymphoid cells from bovine afferent (peripheral) lymph.Immunology. 1987 Oct;62(2):177-83. Immunology. 1987. PMID: 2960610 Free PMC article.
-
Theileria annulata and T. parva infect and transform different bovine mononuclear cells.Immunology. 1989 Feb;66(2):284-8. Immunology. 1989. PMID: 2784413 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources