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. 1980 Nov;87(2 Pt 1):326-35.
doi: 10.1083/jcb.87.2.326.

A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells

W Z Cande

A permeabilized cell model for studying cytokinesis using mammalian tissue culture cells

W Z Cande. J Cell Biol. 1980 Nov.

Abstract

PtK1 cells lysed late in cell division in a medium containing the nonionic detergent Brij 58 and polyethylene glycol with continue to undergo cleavage after lysis. Maintenance of cleavage after lysis is dependent on the composition of the lysis medium; the pH must be around neutrality, MgATP must be present, and the free Ca++ concentration should be 1 microM for optimal constriction to occur. Cleavage can be stopped and reinitiated by raising and lowering the Ca++ levels in the lysis medium. Cleavage in the permeabilized cell is blocked by addition of phalloidin, cytochalasin B, and N-ethylmaleimide-modified myosin subfragment-1 to the lysis medium. This represents the first cell model system for studying cleavage since the pioneering studies of Hoffman-Berling in 1954.

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References

    1. Biochim Biophys Acta. 1978 Jul 17;541(4):459-66 - PubMed
    1. J Cell Biol. 1980 Sep;86(3):858-65 - PubMed
    1. J Cell Biol. 1972 May;53(2):579-81 - PubMed
    1. Biochim Biophys Acta. 1954 Nov;15(3):332-9 - PubMed
    1. J Cell Biol. 1977 Jul;74(1):251-63 - PubMed

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