Simultaneous monoamine histofluorescence and neuropeptide immunocytochemistry: II. Correlative distribution of catecholamine varicosities and magnocellular neurosecretory neurons in the rat supraoptic and paraventricular nuclei

Abstract

The comparative morphology of catecholamine (CA) varicosities and neurophysin (NP)-containing perikarya of the supraoptic (SON) and paraventricular nuclei (PVN) was examined. The major CA innervation to the SON and PVN did not coexist with the major distribution of magnocellular perikarya, but was located peripheral to the nuclei. A dense distribution of CA varicosities was found ventral to the neurosecretory perikarya of the SON and overlapped numerous immunoreactive oxytocin- and vasopressin-containing neuritic profiles. Examination of Golgi-stained sections revealed that dendrites from SON perikarya projected to the CA zone and were likely candidates for the processes identified immunocytochemically. In addition, a heterogenous distribution of axosomatic contacts was found within the SON which suggested a preferential innervation of VP-containing neurons. The densest concentration of CA varicosities in the PVN occurred in the periventricular region adjacent to the third ventricle and in the contiguous parvocellular portion of the PVN. These CA varicosities overlapped scattered oxytocinerigic perikarya in both areas. In addition the ventromedial as well as the dorsolateral subnuclei of the PVN were contacted by CA varicosities; this heterogeneous distribution suggests that the each subnucleus of the PVN with its individual hypothalamic, neurohypophyseal, brainstem, or cortical projections may possibly receive a catecholaminergic innervation by a select group of CA cells or nuclear groups from the brain stem.