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. 1980 Jul;77(7):4147-51.
doi: 10.1073/pnas.77.7.4147.

Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates

R Cortese, R Harland, D Melton

Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates

R Cortese et al. Proc Natl Acad Sci U S A. 1980 Jul.

Abstract

The expression of cloned tRNA genes has been studied by injecting single-stranded and double-stranded DNA templates into Xenopus oocyte nuclei. In both forms the genes are faithfully transcribed after injection. Some single-stranded DNA is converted into double-stranded DNA in the oocyte nucleus. This conversion is necessary for the expression of the injected tRNA gene: no tRNA transcription is observed when DNA synthesis is inhibited. We conclude that single-stranded DNA does not serve as a template for faithful transcription of this gene in injected oocytes.

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