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Comparative Study
. 1980;37(2):133-8.
doi: 10.1016/0022-1759(80)90198-2.

Improved cell surface radioiodination of macrophages

A Kulczycki Jr, V Krause, C C Killion, J P Atkinson
Comparative Study

Improved cell surface radioiodination of macrophages

A Kulczycki Jr et al. J Immunol Methods. 1980.

Abstract

Rabbit alveolar macrophages at high cell concentrations are inefficiently radiolabeled by the usual lactoperoxidase-catalyzed iodination method. Soluble macromolecules secreted by macrophages were found to inhibit the radioiodination of macrophages and other cells. A modified procedure is described which minimizes the presence of such inhibitory material and thereby considerably improves the radioiodination efficiency for both alveolar macrophages and the macrophage-like P388D1 cell line.

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