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. 1980 Mar 11;8(5):1043-59.
doi: 10.1093/nar/8.5.1043.

Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae

D Gallwitz, R Seidel
Free PMC article

Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae

D Gallwitz et al. Nucleic Acids Res. .
Free PMC article

Abstract

Two overlapping DNA fragments from yeast Saccharomyces cerevisiae containing the actin gene have been inserted into pBR322 and cloned in E.coli. Clones were identified by hybridization to complementary RNA from a plasmid containing a copy of Dictyostelium actin mRNA. One recombinant plasmid obtained (pYA102) contains a 3.93-kb Hindlll fragment, the other (pYA208) a 5.1-kb Pstl fragment, both share a common 2.2-kb fragment harboring part of the actin gene. Cloned yeast actin DNA was identified by R-loop formation and translation of the hybridized actin mRNA and by DNA sequence analysis. Cytoplasmic actin mRNA has been estimated to be about 1250 nucleotides long. There is only one type of the actin gene in S.cerevisiae.

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References

    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 - PubMed
    1. Biochemistry. 1977 Dec 27;16(26):5801-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 - PubMed
    1. Gene. 1977;2(2):95-113 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Feb;75(2):588-99 - PubMed

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