[Cytophotometric study of the sex chromatin in the giant cell nuclei of the rabbit trophoblast]

Abstract

A cytophotometrical study was made of DNA contents in the giant cell nuclei of rabbit trophoblast and in the Barr bodies separately found in these nuclei, on the 13st day of embryonic development. It was established that the population of trophoblast cells was not homogeneous; polyploid nuclei with 32c and 64c dominating. The DNA content in the sex chromatin body increased with the magnifying of the polyploidy degree; the dependence between the Barr body DNA content and the degree of polyploidy in the nuclei was almost directly proportional. A deflexion from the direct proportional dependence was revealed mainly for the nuclei with the high degree of polyploidy: 128c and 256c. Possible reasons of these deflexions are discussed.