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Comparative Study
. 1980 Nov-Dec;16(6):936-9.

[Rapid method for determining protein concentrations in the cells of microorganisms]

[Article in Russian]
I A Gorina, V I Iakovleva
  • PMID: 7005891
Comparative Study

[Rapid method for determining protein concentrations in the cells of microorganisms]

[Article in Russian]
I A Gorina et al. Prikl Biokhim Mikrobiol. 1980 Nov-Dec.

Abstract

The method of Lowry as modified by Peterson (who had changed concentrations of reagents and added a new reagent, sodium dodecyl sulfate) has been tested. It has been shown that the modified method can be applied for rapid measurement of protein in the suspension of bacterial cells. The effect of the following compounds on the protein estimation by the modified method in bovine serum albumin has been studied: potassium chloride, potassium phosphate, sucrose, EDTA, tris, ammonium acetate, Triton X-100, tyrosine, phenol, glycerol, 2-mercaptoethanol.

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