Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1980 Nov;77(11):6329-33.
doi: 10.1073/pnas.77.11.6329.

Autonomously replicating sequences in Saccharomyces cerevisiae

C S Chan, B K Tye

Autonomously replicating sequences in Saccharomyces cerevisiae

C S Chan et al. Proc Natl Acad Sci U S A. 1980 Nov.

Abstract

A method is presented for isolating DNA segments capable of autonomous replication from Saccharomyces cerevisiae chromosomal DNA based on the differential transforming ability of autonomously replicating plasmids and nonreplicating plasmids. DNA plasmids that are capable of self-replication in yeast transform yeast spheroplasts at about 1000-fold higher frequency than nonreplicating plasmids. We have cloned from total yeast DNA a number of DNA segments that permit the pBR322 plasmid carrying the yeast LEU2 gene to replicate autonomously. These plasmid clones are characterized by their ability to transform Leu- spheroplasts to Leu+ at a high frequency and their ability to replicate autonomously. Analysis of the insert DNAs carried in some of these self-replicating plasmids divides them into two categories: those that are unique in the yeast genome and those that are repetitive.

PubMed Disclaimer

References

    1. Science. 1968 Aug 9;161(3841):529-40 - PubMed
    1. J Mol Biol. 1969 Mar 14;40(2):261-77 - PubMed
    1. J Biol Chem. 1970 Jul 25;245(14):3626-31 - PubMed
    1. J Bacteriol. 1972 May;110(2):667-76 - PubMed
    1. J Bacteriol. 1973 Nov;116(2):1064-6 - PubMed

Publication types