doi: 10.1111/j.1432-1033.1980.tb07208.x.
Activity determination of 3-iodopyridineadenine dinucleotide and its phosphate as hydride acceptors in the presence of dehydrogenases using a coupled redox system
- PMID: 7007042
- DOI: 10.1111/j.1432-1033.1980.tb07208.x
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Activity determination of 3-iodopyridineadenine dinucleotide and its phosphate as hydride acceptors in the presence of dehydrogenases using a coupled redox system
Eur J Biochem.
1980 Nov.
Free article
Abstract
A new procedure for the activity measurement of NAD(P)+-dependent dehydrogenases has been devised using an electron-transferring agent, phenazine methosulfate, and an electron acceptor, 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide. The reduction of the latter is determined by an increase in absorbance at 578 nm. 3-Iodopyridineadenine dinucleotide was found to be active as an hydride acceptor with horse liver alcohol dehydrogenase and lactate dehydrogenase but showed no activity with glyceraldehyde-3-phosphate dehydrogenase nor did its phosphate with 3-phosphogluconate dehydrogenase.
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