Molecular cloning of the K1 capsular polysaccharide genes of E. coli

Abstract

Epidemiological and immunological evidence indicates that the K1 capsular polysaccharide confers the property of virulence on Escherichia coli. E coli K1 is associated with invasive diseases in humans and in laboratory and domesticated animals. K1 isolates account for 80% of E. coli neonatal meningitis and comprise the majority of capsular types in neonatal septicaemia without meningitis and in childhood pyelonephritis. Passive administration of K1 antibodies prevented bacteraemia and meningitis in infant rats fed E. coli K1. Nonencapsulated derivatives of these invasive K1 strains did not cause bacteraemia in infant rats, although intestinal colonization was similar to that of the parent strains (M. Achtman and R.P.S., unpublished results). Several reports propose that the E. coli K1 capsular polysaccharide exerts an anti-phagocytic effect similar to that observed with other pathogenic encapsulated bacteria. One approach to studying whether the K1 antigen is sufficient to confer virulence of if other E. coli structures are necessary is to isolate the K1 genes for genetic and biochemical analysis. Recombinant DNA methodology provides a powerful tool for such an approach. Here, we report the molecular cloning of the E. coli K1 antigen genes. The cloned K1 genes synthesize a capsule in E. coli K12 indistinguishable chemically and immunologically from that of wild-type K1 strains.