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. 1980 Dec 11;8(23):5725-37.
doi: 10.1093/nar/8.23.5725.

Yeast histone mRNA is polyadenylated

K Fahrner, J Yarger, L Hereford
Free PMC article

Yeast histone mRNA is polyadenylated

K Fahrner et al. Nucleic Acids Res. .
Free PMC article

Abstract

Histone mRNA from S. cerevisiae has been identified and partially purified. The RNA is quantitatively retained on oligo (dT) cellulose or poly(U) sepharose as assayed by in vitro translation or hybridization of radiolabelled cloned yeast histone sequences to RNA immobilized on DBM paper. Retention of yeast histone mRNA on either of these chromatographic systems is most likely the result of polyadenylation since, when primed with oligo (dT), the RNA is an extremely good template for reverse transcriptase, as determined by hybrid arrest translation or by hybridization to D. melanogaster histone DNA sequences.

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