Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Apr;146(1):422-5.
doi: 10.1128/jb.146.1.422-425.1981.

Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli

S Brown, E R Brickman, J Beckwith

Blue ghosts: a new method for isolating amber mutants defective in essential genes of Escherichia coli

S Brown et al. J Bacteriol. 1981 Apr.

Abstract

We describe a technique which permits an easy screening for amber mutants defective in essential genes of Escherichia coli. Using this approach, we have isolated three amber mutants defective in the rho gene. An extension of the technique allows the detection of ochre mutants and transposon insertions in essential genes.

PubMed Disclaimer

References

    1. J Bacteriol. 1971 Feb;105(2):455-66 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158-62 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1112-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 May;72(5):1725-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3238-42 - PubMed

Publication types

LinkOut - more resources