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. 1981 May 10;256(9):4676-8.

A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli

M M Cox, K McEntee, I R Lehman
  • PMID: 7012155
Free article

A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli

M M Cox et al. J Biol Chem. .
Free article

Abstract

A simple and rapid three-step procedure for the large scale purification of the recA protein of Escherichia coli is described. The method depends primarily on a single chromatographic step which is highly specific for recA protein: elution by ATP from single-stranded DNA cellulose. With this procedure, gram quantities of recA protein, greater than 99% pure, can be reproducibility prepared for biochemical and biophysical analysis.

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