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. 1978 Oct;79(1):281-5.
doi: 10.1083/jcb.79.1.281.

Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels

W S Adair, D Jurivich, U W Goodenough

Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels

W S Adair et al. J Cell Biol. 1978 Oct.

Abstract

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.

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References

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