A method for electron microscopic preparation of cultured cells (monolayer) in a new test chamber (TCSC-1)

Abstract

A time-saving method is described for the preparation of cultivated cells (monolayer) for electron microscopy using a new test chamber system (TCSC-1). The versatile chamber is fitted with a screw-in PTFE-adapter which connects via a silicone ring gasket to the surface on which the cells grown. All procedures (fixation, staining, embedding) are performed directly in the chamber. The cells, in this particular communication epithelial liver cells, are grown as a monolayer on polyester foils. After embedding of the cells, the foil can be easily taken off. Removal of the foil does not in any way effect the quality of the first slice for electron microscopic examination. The resin block can also be easily pressed out from the PTFE-adapter.