doi: 10.1038/291117a0.
Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase
- PMID: 7015143
- DOI: 10.1038/291117a0
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Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase
Nature.
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Abstract
Synthesis of the large subunit polypeptide of ribulose bisphosphate carboxylase can be detected in Escherichia coli cells containing the chloroplast genes from maize and wheat. The chloroplast DNA sequences contain a 'promoter' that, in Escherichia coli, initiates transcription and translation of the genes. This synthesis of large subunits has been substantially increased using the powerful lambda promoter, PL, to drive transcription of the maize gene.
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