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. 1981 May 14;291(5811):117-21.
doi: 10.1038/291117a0.

Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase

A A Gatenby, J A Castleton, M W Saul

Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase

A A Gatenby et al. Nature. .

Abstract

Synthesis of the large subunit polypeptide of ribulose bisphosphate carboxylase can be detected in Escherichia coli cells containing the chloroplast genes from maize and wheat. The chloroplast DNA sequences contain a 'promoter' that, in Escherichia coli, initiates transcription and translation of the genes. This synthesis of large subunits has been substantially increased using the powerful lambda promoter, PL, to drive transcription of the maize gene.

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