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. 1981 Feb 25;9(4):831-9.
doi: 10.1093/nar/9.4.831.

The characterisation of a 5-S 'monosome' fraction from chromatin of Physarum polycephalum

M Annesley, K E Davies, N M Kumar, I O Walker
Free PMC article

The characterisation of a 5-S 'monosome' fraction from chromatin of Physarum polycephalum

M Annesley et al. Nucleic Acids Res. .
Free PMC article

Abstract

A so-called '5-S mononucleosome' fraction was isolated from nuclei of Physarum polycephalum by digestion with micrococcal nuclease and subsequent fractionation by gel filtration. This fraction had electrophoretic and sedimentation properties which were similar to DNA of approximately 140 base pairs in length. It is shown that lysis of the nuclei activates a protease which is resistant to phenyl-methyl sulphonyl fluoride, o-phenanthroline and parachloromercuri benzene sulphonate and which subsequently degrades the nucleosomes.

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