Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Feb;78(2):922-5.
doi: 10.1073/pnas.78.2.922.

Molecular basis of valine resistance in Escherichia coli K-12

R P Lawther, D H Calhoun, C W Adams, C A Hauser, J Gray, G W Hatfield

Molecular basis of valine resistance in Escherichia coli K-12

R P Lawther et al. Proc Natl Acad Sci U S A. 1981 Feb.

Abstract

The relationship of valine resistance to the expression of the ilvGEDA operon of Escherichia coli K-12 has been determined. DNA sequence and in vivo protein analyses indicate that in wild-type E. coli K-12 there is a frameshift site within the gene (ilvG) for valine resistance. The ilvG+2096 (formerly designated ilv02096) mutation displaces this frameshift site, resulting in the expression of ilvG and the relief of transcriptional polarity on the distal genes of this operon. Thus, the "ilv0" mutation, which concomitantly confers valine resistance and increased expression of the ilvEDA genes, is, in fact, the "reversion" of a polar site within the first structural gene of the ilvGEDA operon.

PubMed Disclaimer

References

    1. Annu Rev Genet. 1979;13:319-53 - PubMed
    1. J Bacteriol. 1974 Dec;120(3):1068-77 - PubMed
    1. Mol Gen Genet. 1976 Feb 2;143(3):243-52 - PubMed
    1. Mol Gen Genet. 1979 Feb 1;169(3):299-314 - PubMed
    1. Genetics. 1976 Jun;83(2):201-25 - PubMed

Publication types

LinkOut - more resources