[ED-20 epoxy resin as an embedding medium for biological objects for electron microscopy study]

Abstract

A method of embedding of biological objects for electron microscopy is described. A solution of epoxide resin ED-20 with a plastifier, dibutyl phthalate, and a solidifier, standard polyethylene polyamine, comprising 8% and 12% of the total mass of resin, was added into capsules into which blocks of tissues to be examined that had been pre-impregnated in increasing resin-in-acetone solution series were placed. Polymerization occurred under conventional conditions at room temperature for 24 hours or at 37 degrees C for 12 hours.