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. 1981 Jun 15;214(2):433-9.
doi: 10.1016/0006-8993(81)91208-7.

Monoclonal antibody against cell surface glycoprotein of neurons

M Hirn, M Pierres, H Deagostini-Bazin, M Hirsch, C Goridis

Monoclonal antibody against cell surface glycoprotein of neurons

M Hirn et al. Brain Res. .

Abstract

A monoclonal antibody, named BSP-2, has been produced against glycoproteins extracted from neonatal mouse brain. Its reactivity with live cells established the surface location of the antigen. In primary cultures of dissociated cerebellar cells, the antibody bound to neuronal cell types, but not to astrocytes nor to fibroblasts. Immunoprecipitates prepared with the BSP-2 antibody contained a triplet of high-molecular weight glycoproteins with apparent molecular weights of 180,000, 140,000 and 120,000.

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