Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1981 Jan;78(1):238-42.
doi: 10.1073/pnas.78.1.238.

Cloning of yeast gene for trichodermin resistance and ribosomal protein L3

H M Fried, J R Warner

Cloning of yeast gene for trichodermin resistance and ribosomal protein L3

H M Fried et al. Proc Natl Acad Sci U S A. 1981 Jan.

Abstract

Yeast cells sensitive to the eukaryotic protein synthesis inhibitor trichodermin have been transformed with autonomously replicating recombinant plasmids carrying DNA fragments of the genome of a trichodermin-resistant yeast strain. After selection for trichodermin-resistant cells, several transformants yielded a plasmid containing a 13.5-kilobase (kb) DNA fragment that encodes the trichodermin resistance gene, tcm1, and the gene for ribosomal protein L3, the largest of the yeast ribosomal proteins. Cells carrying this plasmid are resistant to trichodermin and to the related drug verrucarin A as well as to the unrelated drug anisomycin. This pattern of resistance is similar to that exhibited by strains carrying a chromosomal copy of tcm1. Moreover, polyribosomes prepared from transformed cells are resistant to trichodermin when tested in an in vitro protein synthesis assay. Subcloning of the 13.5-kb DNA fragment revealed that the gene for tcm1 and the gene for protein L3 are contained within a 3.2-kb segment. These results suggest that the gene for trichodermin resistance in yeast specifies ribosomal protein L3.

PubMed Disclaimer

References

    1. Methods Cell Biol. 1978;20:45-60 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 - PubMed
    1. Somatic Cell Genet. 1978 May;4(3):355-74 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Jan;76(1):410-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1035-9 - PubMed

Publication types

MeSH terms

LinkOut - more resources