Effects of O2 stress on tomato alcohol dehydrogenase activity: description of a second ADH coding genes

Abstract

Subjecting tomato seedlings to anaerobic conditions results in expression of a previously undescribed Adh gene, Adh-2. Induction profiles were similar for all tissues, including roots, hypocotyls, cotyledons, and true leaves. In sharp contrast to ADH-1, ADH-2 showed no induction under anaerobic stress. The only time ADH-2 activity was expressed (under noninduced conditions) was during the early stages of embryogenesis. By late embryogenesis, ADH-2 activity approached a zero level, concomitant with a sharp rise in ADH-1 activity, which is found in the cotyledons of quiescent embryo. Despite striking differences in the regulation of these two genes, their homology is demonstrated in the ability of their enzyme subunits to form presumed intergenic heterodimers, which are visible during the transient period of embryogenesis when the polypeptides encoded by both genes are expressed. A multiple point linkage test using isozymic marker genes placed the Adh-2 locus on chromosome 6 near Aps-1, whereas Adh-1 resides on chromosome 4.