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. 1981;43(3):269-75.
doi: 10.1016/0022-1759(81)90174-5.

Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies

C J Yeh, B L Hsi, W P Faulk

Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies

C J Yeh et al. J Immunol Methods. 1981.

Abstract

The use of a fluorescence method employing propidium iodide to determine nuclear morphology of different cell types by epi-illumination in membrane immunofluorescence is described. Its usefulness is illustrated by differentiating nucleated and anuclear transferrin receptor-bearing cells as well as by simultaneously determining the viability of cell suspension without requiring a change from fluorescence microscopy, this causing no loss of visual accommodation.

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