doi: 10.1016/0022-1759(81)90174-5.
Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies
- PMID: 7019344
- DOI: 10.1016/0022-1759(81)90174-5
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Propidium iodide as a nuclear marker in immunofluorescence. II. Use with cellular identification and viability studies
J Immunol Methods.
1981.
Abstract
The use of a fluorescence method employing propidium iodide to determine nuclear morphology of different cell types by epi-illumination in membrane immunofluorescence is described. Its usefulness is illustrated by differentiating nucleated and anuclear transferrin receptor-bearing cells as well as by simultaneously determining the viability of cell suspension without requiring a change from fluorescence microscopy, this causing no loss of visual accommodation.
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