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. 1981 Jul;34(7):712-8.
doi: 10.1136/jcp.34.7.712.

Cultural method for large-scale screening for Chlamydia trachomatis genital infection

H Mallinson, S Sikotra, O P Arya

Cultural method for large-scale screening for Chlamydia trachomatis genital infection

H Mallinson et al. J Clin Pathol. 1981 Jul.

Abstract

Idoxuridine-treated McCoy cells grown as monolayers in 96 well microplates provide a convenient method for the isolation of Chlamydia trachomatis. Staining of infected monolayers with periodic acid-Schiff reagent (PAS) allows easy recognition of C trachomatis inclusions without the need for dark-ground microscopy. By this method 384 clinical specimens can be examined concurrently. It is sufficiently sensitive to form the basis of a chlamydial culture service for patients attending Sexually Transmitted Diseases (STD) Clinics.

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