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. 1981;21(3):181-9.
doi: 10.1002/jobm.3630210303.

Study of inulase from Debaryomyces phaffii Capriotti

Study of inulase from Debaryomyces phaffii Capriotti

S Demeulle et al. Z Allg Mikrobiol. 1981.

Abstract

Debaryomyces phaffii possesses an inulinase activity which can be induced by growth on beta-fructosidase and particularly on inulin. The enzyme is located in the cell wall but is easily excreted into the culture medium. Maximum activity on inulin is observed at pH 4 and 50 degrees C. The Km on inulin is 1.2 X 10(-2) M. The enzyme breaks down inulin by splitting off terminal fructosyl units and it is active on sucrose and raffinose.

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