Use of monoclonal antihapten antibodies for immunolocalisation of tissue antigens
- PMID: 7024320
- PMCID: PMC494225
- DOI: 10.1136/jcp.34.9.1000
Use of monoclonal antihapten antibodies for immunolocalisation of tissue antigens
Abstract
The applicability of a hapten-antihapten sandwich technique incorporating a monoclonal antihapten bridge antibody to localisation of tissue antigen is described. The results show that the method is both versatile and selective and has the potential of being even more sensitive than currently used immunoperoxidase methods.
Similar articles
-
Separation of monoclonal antibodies from antihapten antisera by two-dimensional affinity electrophoresis.J Chromatogr. 1992 Apr 24;597(1-2):365-76. doi: 10.1016/0021-9673(92)80133-f. J Chromatogr. 1992. PMID: 1517340
-
Hapten-sandwich labeling. III. Bifunctional reagents for immunospecific labeling of cell surface antigens.J Immunol. 1976 Nov;117(5 Pt 1):1695-1704. J Immunol. 1976. PMID: 1002997
-
Hapten-sandwich labeling. IV. Improved procedures and non-cross-reacting hapten reagents for double-labeling cell surface antigens.J Immunol Methods. 1979;25(3):283-9. doi: 10.1016/0022-1759(79)90116-9. J Immunol Methods. 1979. PMID: 368251
-
Dinitrophenyl (DNP) hapten sandwich staining (DHSS) procedure. A 10 year review of its principle reagents and applications.J Immunol Methods. 1992 Jun 24;150(1-2):193-8. doi: 10.1016/0022-1759(92)90078-8. J Immunol Methods. 1992. PMID: 1319440 Review.
-
Kinetics of antibody--hapten reactions.Contemp Top Mol Immunol. 1975;4:23-54. Contemp Top Mol Immunol. 1975. PMID: 209 Review. No abstract available.
Cited by
-
Histological demonstration of immunoreactive zinc metallothionein in liver and ileum of rat and man.Histochem J. 1985 Mar;17(3):343-52. doi: 10.1007/BF01004596. Histochem J. 1985. PMID: 3897145
-
Lectin--digoxigenin conjugates: a new hapten system for glycoconjugate cytochemistry.Histochemistry. 1990;94(1):1-11. doi: 10.1007/BF00266783. Histochemistry. 1990. PMID: 1693608
-
The use of vimentin antibodies in the diagnosis of malignant mesothelioma.Virchows Arch A Pathol Anat Histopathol. 1985;406(4):441-8. doi: 10.1007/BF00710235. Virchows Arch A Pathol Anat Histopathol. 1985. PMID: 3925619
-
Immunological events leading to destructive thyroiditis in the AUG rat.Clin Exp Immunol. 1988 Sep;73(3):410-6. Clin Exp Immunol. 1988. PMID: 3208452 Free PMC article.
-
Biological markers in human lung carcinoma: an immunopathological study of six antigens.Thorax. 1983 Dec;38(12):937-41. doi: 10.1136/thx.38.12.937. Thorax. 1983. PMID: 6198740 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources