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. 1978 Oct;28(1):408-10.
doi: 10.1128/JVI.28.1.408-410.1978.

Identification of lysis protein E of bacteriophage phiX174

Identification of lysis protein E of bacteriophage phiX174

T J Pollock et al. J Virol. 1978 Oct.

Abstract

The product of gene E, the lysis gene of phiX174, has been identified as a distinct band in a sodium dodecyl sulfate-gel electropherogram. The position of the band is consistent with the molecular weight of 10,589 calculated from the nucleotide sequence of the gene. The band is eliminated by a nonsense mutation in gene E. It is estimated that roughly 100 to 300 molecules of E protein are made in an infected cell; this appears to be less than one-tenth the amount of protein made by gene D, in which gene E is wholly contained.

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References

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