Identification of lysis protein E of bacteriophage phiX174
- PMID: 702655
- PMCID: PMC354281
- DOI: 10.1128/JVI.28.1.408-410.1978
Identification of lysis protein E of bacteriophage phiX174
Abstract
The product of gene E, the lysis gene of phiX174, has been identified as a distinct band in a sodium dodecyl sulfate-gel electropherogram. The position of the band is consistent with the molecular weight of 10,589 calculated from the nucleotide sequence of the gene. The band is eliminated by a nonsense mutation in gene E. It is estimated that roughly 100 to 300 molecules of E protein are made in an infected cell; this appears to be less than one-tenth the amount of protein made by gene D, in which gene E is wholly contained.
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