X-ray scattering from randomly oriented superhelices. Circular superhelical DNA

Abstract

The scattering functions of randomly oriented filaments of finite length exhibiting two orders of helicity have been calculated. It is shown to a good approximation that each order scatters as if present alone as a first order helix of the same contour length and pitch angle. These results show that the measured scattering pattern from dissolved superhelical DNA molecules is consistent with the scattering pattern calculated for a coiled coil geometry.