doi: 10.1093/nar/9.20.5469.
Determination of DNA cooperativity factor
- PMID: 7029470
- PMCID: PMC327533
- DOI: 10.1093/nar/9.20.5469
Item in Clipboard
Determination of DNA cooperativity factor
Nucleic Acids Res.
.
Abstract
The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5x10(-5) for the cooperatively factor sigma. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.
Similar articles
-
Differential scanning calorimetric and theoretical studies of ColE1 DNA.Nucleic Acids Symp Ser. 1986;(17):203-6. Nucleic Acids Symp Ser. 1986. PMID: 3550711
-
Relationship between helix-coil transition and gene organization of ColE1 plasmid DNA. Differential scanning calorimetric and theoretical studies.J Mol Biol. 1987 Apr 20;194(4):691-8. doi: 10.1016/0022-2836(87)90246-4. J Mol Biol. 1987. PMID: 3656401
-
Equilibrium melting of plasmid ColE1 DNA: electron-microscopic visualization.Nucleic Acids Res. 1980 Sep 25;8(18):4165-84. doi: 10.1093/nar/8.18.4165. Nucleic Acids Res. 1980. PMID: 6253910 Free PMC article.
-
The ionic strength dependence of the cooperativity factor for DNA melting.J Biomol Struct Dyn. 1987 Aug;5(1):119-26. doi: 10.1080/07391102.1987.10506380. J Biomol Struct Dyn. 1987. PMID: 3271461
-
[Molecular melting of DNA and the effect of the fine structure of fusion curves].Mol Biol (Mosk). 1977 Nov-Dec;11(6):1311-24. Mol Biol (Mosk). 1977. PMID: 377064 Review. Russian.
Cited by
-
Sequence-dependent base pair opening in DNA double helix.Biophys J. 2006 May 1;90(9):3091-9. doi: 10.1529/biophysj.105.078774. Epub 2006 Feb 24. Biophys J. 2006. PMID: 16500982 Free PMC article.
-
Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.Crit Rev Eukaryot Gene Expr. 2016;26(1):63-96. doi: 10.1615/CritRevEukaryotGeneExpr.v26.i1.70. Crit Rev Eukaryot Gene Expr. 2016. PMID: 27278886 Free PMC article. Review.
-
Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application.Nucleic Acids Res. 1982 Aug 11;10(15):4813-26. doi: 10.1093/nar/10.15.4813. Nucleic Acids Res. 1982. PMID: 7133999 Free PMC article.
-
The nonequilibrium character of DNA melting: effects of the heating rate on the fine structure of melting curves.Nucleic Acids Res. 1984 May 25;12(10):4339-49. doi: 10.1093/nar/12.10.4339. Nucleic Acids Res. 1984. PMID: 6328418 Free PMC article.
-
The effect of nucleotide substitution on DNA denaturation profiles.Nucleic Acids Res. 1987 Aug 25;15(16):6665-76. doi: 10.1093/nar/15.16.6665. Nucleic Acids Res. 1987. PMID: 2819824 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
