Simple method for isolation and presumptive identification of Clostridium difficile
- PMID: 7032146
Item in Clipboard
Simple method for isolation and presumptive identification of Clostridium difficile
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol.
1981.
Abstract
Clostridium difficile can be isolated from stools and presumptively identified by inoculating the stools onto CCCBA and the culture looked at under ultra violet light where the golden-yellow fluorescent colonies show up against a dark background. The colonies are then identified by testing them using the API ZYM system and the results further confirmed by testing the culture for toxin production using the tissue culture technique employing Cl. sordellii antitoxin.
Similar articles
-
Cultures for Clostridium difficile in stools containing a cytotoxin neutralized by Clostridium sordellii antitoxin.J Clin Microbiol. 1979 Dec;10(6):880-4. doi: 10.1128/jcm.10.6.880-884.1979. J Clin Microbiol. 1979. PMID: 521486 Free PMC article.
-
[Enumeration and identification of Clostridium from stools treated with the thioglycollate-lysozyme method].Arch Inst Pasteur Alger. 1989;57:277-84. Arch Inst Pasteur Alger. 1989. PMID: 2489405 French.
-
Detection of Clostridium difficile toxins in stools. Comparison between a new enzyme immunoassay for toxin A and other routine tests.Gastroenterol Clin Biol. 1993;17(4):283-6. Gastroenterol Clin Biol. 1993. PMID: 8339887
-
Rapid techniques for the identification of anaerobic bacteria and presumptive diagnosis.Scand J Infect Dis Suppl. 1982;35:23-30. Scand J Infect Dis Suppl. 1982. PMID: 6188205 Review. No abstract available.
-
The role of the clinical microbiology laboratory in the management of Clostridium difficile-associated diarrhea.Infect Dis Clin North Am. 1993 Jun;7(2):277-93. Infect Dis Clin North Am. 1993. PMID: 8345170 Review.
Cited by
-
Use of cycloserine-cefoxitin-fructose agar and L-proline-aminopeptidase (PRO Discs) in the rapid identification of Clostridium difficile.J Clin Microbiol. 1997 May;35(5):1258-9. doi: 10.1128/jcm.35.5.1258-1259.1997. J Clin Microbiol. 1997. PMID: 9114419 Free PMC article.
-
Incidence and origin of Clostridium difficile in neonates.J Clin Microbiol. 1984 Jan;19(1):77-8. doi: 10.1128/jcm.19.1.77-78.1984. J Clin Microbiol. 1984. PMID: 6690469 Free PMC article.
-
Evaluation of the ATB 32 A system for identification of anaerobic bacteria isolated from clinical specimens.J Clin Microbiol. 1990 Jul;28(7):1519-24. doi: 10.1128/jcm.28.7.1519-1524.1990. J Clin Microbiol. 1990. PMID: 2199516 Free PMC article.
-
Clostridium difficile: its disease and toxins.Clin Microbiol Rev. 1988 Jan;1(1):1-18. doi: 10.1128/CMR.1.1.1. Clin Microbiol Rev. 1988. PMID: 3144429 Free PMC article. Review.
-
Identification of Clostridium difficile using the API ZYM system.Eur J Clin Microbiol. 1985 Oct;4(5):505-7. doi: 10.1007/BF02014434. Eur J Clin Microbiol. 1985. PMID: 4065138